Purification and characterization of cytosolic 6-phosphogluconate dehydrogenase isozymes from maize.
نویسندگان
چکیده
Cytosolic isozymes of 6-phosphogluconate dehydrogenase were purified from roots of maize (Zea mays L.). The final preparation contained two 55-kD proteins. Affinity-purified dehydrogenases from a maize line that is null for both cytosolic 6-phosphogluconate dehydrogenase isozymes (Pgd1-null, Pgd2-null) lacked the 55-kD proteins. The substrate kinetics of the purified enzyme were determined.
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ورودعنوان ژورنال:
- Plant physiology
دوره 100 3 شماره
صفحات -
تاریخ انتشار 1992